Human IL-4 (Interleukin 4) ELISPOT Kit - ABMIUM
Manufactured by:Elabscience
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Human IL-4 (Interleukin 4) ELISPOT Kit

Human IL-4 (Interleukin 4) ELISPOT Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM13002-1
Product type
Assay Kits
TargetIL-4

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human IL-4 (Interleukin 4) ELISPOT Kit from Elabscience is intended for research workflows involving IL-4. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Cell Biology, Immunology
Also known as
IL4,BCGF-1,BCGF1,BSF-1,BSF1
Cross-reactivity
This kit recognizes Human IL-4 in samples.No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
Incubation temperature
2-8℃
Assay type
ELISPOT Kits
Technical note
This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-4 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-4 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-4 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-4 antibodies will bind specifically to the IL-4 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-4 secreting cells.
Identifiers & database references
Supplier SKU / catalogue number
ESP-H0007_96T
Gene symbol
IL-4
Gene ID
3565
UniProt accession
P05112
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£313.00£250.40