11β-HSD1 rabbit pAb from ELK Biotechnology Read more ›
11β-HSD1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying 11β-HSD1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
hydroxysteroid 11-beta dehydrogenase 1(HSD11B1) Homo sapiens The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011],
| Specificity | 11β-HSD1 Polyclonal Antibody detects endogenous levels of 11β-HSD1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 cells using 11β-HSD1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100 |
| Collaborator | ELK Biotechnology |
| Partner model | ES4290 |
| Target | 11β-HSD1 |
| Research areas | >>Steroid hormone biosynthesis, >>Metabolism of xenobiotics by cytochrome P450, >>Metabolic pathways, >>Chemical carcinogenesis - DNA adducts |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from 11β-HSD1 . at AA range: internal |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed, highest expression in liver, lower in testis, ovary, lung, foreskin fibroblasts, and much lower in kidney (PubMed:1885595). Expressed in liver (at protein level) (PubMed:21453287). Expressed in the basal cells of the corneal epithelium and in the ciliary nonpigmented epithelium (both at mRNA and at protein level) (PubMed:11481269). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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