Kit ELISA Microsample APOB (Apolipoprotéine B) de rat de ELK Biotechnology
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Le kit ELISA pour microéchantillons de l'APOB (apolipoprotéine B) de rat est un kit ELISA de biotechnologie ELK destiné à la mesure de l'APOB à des fins de recherche.
Le principe de test appliqué dans ce kit est l'immuno-essai enzymatique de type Sandwich. La plaque de microtitration fournie dans ce kit a été pré-enduite d'un anticorps spécifique de l'APOB de rat. Des étalons ou des échantillons sont ajoutés aux puits appropriés de la plaque de microtitration, puis un anticorps conjugué à la biotine spécifique de l'APOB de rat est ajouté. Ensuite, de l'Avidine conjuguée à la peroxydase de raifort (HRP) est ajoutée à chaque puits de la microplaque et incubée. Après l'ajout de la solution de substrat TMB, seuls les puits contenant l'APOB de rat, l'anticorps conjugué à la biotine et l'Avidine conjuguée à l'enzyme présenteront un changement de couleur. La réaction enzyme-substrat est terminée par l'ajout d'une solution d'acide sulfurique et le changement de couleur est mesuré par spectrophotométrie à une longueur d'onde de 450 nm ± 10 nm. La concentration d'APOB de rat dans les échantillons est ensuite déterminée en comparant la DO des échantillons à la courbe étalon.
Les noms alternatifs incluent Apo-B ; FLDB ; Apo B-100 ; Apo B-48.
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Sérum | Plasma | Homogénats tissulaires | Lysats cellulaires | Surnageants de culture cellulaire | Autres fluides biologiques |
|---|---|---|---|---|---|---|
| Compatibilité de l'échantillon |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
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Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
APOB (apolipoprotein B) encodes the major structural apolipoprotein of chylomicrons and low-density lipoproteins. Human APOB gives rise to two principal plasma forms: ApoB-100, synthesized mainly in the liver and acting as a ligand for the LDL receptor, and ApoB-48, produced in the intestine through RNA editing. Researchers use APOB to investigate lipoprotein particle biology, lipid transport and receptor-mediated clearance.
Biological function: Apolipoprotein B provides the core protein scaffold for major circulating lipoprotein particles. ApoB-100 is the full-length hepatic form found in LDL and functions as a recognition ligand for LDL-receptor-mediated particle uptake. ApoB-48 is the shorter intestinal form in chylomicrons and is generated from the same APOB transcript by RNA editing that introduces an early stop codon. These forms link APOB to transport of triglyceride- and cholesterol-rich particles between tissues.
