Custom research services · ABMIUM
Antibody Engineering & Humanisation
Antibody humanisation, sequencing, recombinant conversion and affinity optimisation. Define your starting clone, format and functional testing with ABMIUM.
Specification reviewed before feasibility, pricing and lead time are confirmed.
Built around the experiment
Your project. A defined technical scope.
Antibody engineering adapts an existing clone or sequence to a new experimental requirement. ABMIUM coordinates projects covering hybridoma sequencing, recombinant expression, antibody format changes and humanisation. The starting sequence, binding assay and required final format determine the scope. Agree how binding and function will be compared with the original material before choosing an engineering strategy.
Specialist ProMab service routes can be coordinated through ABMIUM. Your quotation identifies the selected provider, agreed stages, available documentation and material or experimental deliverables.
ABMIUM reviews your specification and coordinates suitable custom-service and manufacturing routes. The proposed provider or manufacturer, available documentation and agreed scope are stated before order.
Choose the stages you need
Service modules
Start with one module or combine related stages into a clearly scoped project.
Hybridoma sequencing
Request identification of antibody variable-region sequences from a supplied hybridoma. Confirm sample condition, clonality, the number of chains to resolve and the...
Technical scope
Request identification of antibody variable-region sequences from a supplied hybridoma. Confirm sample condition, clonality, the number of chains to resolve and the desired sequence deliverable. Sequence recovery can support recombinant conversion, construct development and continuity of a research antibody.
Recombinant conversion and reformatting
Convert a characterised antibody sequence into an agreed recombinant format such as IgG, Fab, scFv or an Fc fusion. Define species, isotype,...
Technical scope
Convert a characterised antibody sequence into an agreed recombinant format such as IgG, Fab, scFv or an Fc fusion. Define species, isotype, tags and purification requirements. Expression and binding checks should be scoped together so the new format can be compared with the original reagent.
Antibody humanisation
Discuss humanisation of non-human variable regions with a plan to assess retention of binding and activity. Provide the parental sequence and any...
Technical scope
Discuss humanisation of non-human variable regions with a plan to assess retention of binding and activity. Provide the parental sequence and any existing functional data. A humanised sequence is an engineered research candidate; clinical suitability is a separate development question.
Affinity optimisation and production
Explore affinity optimisation against a defined antigen and assay objective. State whether the goal is improved binding, altered kinetics or preserved specificity....
Technical scope
Explore affinity optimisation against a defined antigen and assay objective. State whether the goal is improved binding, altered kinetics or preserved specificity. Research-scale expression, purification and material supply can be added, with QC and comparison assays defined in the quotation.
Connect the service to your research
Research applications
Clone continuity
Move a research clone toward a sequence-defined supply route and specify the documentation needed for future production.
Format and assay comparison
Compare fragment, isotype or Fc changes using agreed binding and functional assays.
Immunotherapy discovery
Prepare sequence and material packages for later bispecific antibody or CAR research, subject to project feasibility.
Agree the output before work begins
Project deliverables
Material, experiments and documentation are defined in the quotation. Select the deliverables that your study needs.
- Heavy- and light-chain sequence information where sequencing is commissioned.
- Engineered constructs or purified antibody material in the agreed format.
- Expression, purification and binding comparison reports within scope.
- Defined handling, QC, ownership and repeat-supply terms.
One coordinated route
How your custom project works
- 01
Share your requirement
Tell us the target, application, starting materials and output you need. Include performance and documentation requirements.
- 02
Review feasibility and scope
ABMIUM coordinates the technical review and identifies the proposed provider, methods, deliverables and project stages.
- 03
Confirm the quotation
Review the agreed specification, available QC and reports, pricing, indicative lead time and delivery terms before order.
Clear answers before you enquire
Frequently asked questions
Can you sequence an existing hybridoma?
Hybridoma sequencing can be scoped through ABMIUM. Provide cell-line details and sample availability so the route and chain-resolution requirements can be assessed.
Can an IgG be converted to an scFv?
Reformatting can be discussed from an antibody sequence. Binding may change with format, so expression and functional comparison should be part of the project plan.
Does humanisation guarantee unchanged binding?
No. Retention of binding and function needs evaluation against the parental antibody. The quotation should state the design and validation stages.
Can engineering and production be quoted together?
Yes. Include the final amount, buffer, purity, application and QC requirements so the engineering and material-supply stages can be scoped together.
How does antibody humanisation (humanization) differ from recombinant conversion?
No. Recombinant conversion expresses a sequence-defined antibody; humanisation changes sequence composition toward a human framework while seeking to retain useful binding. Provide the parent sequence or source material, intended format and functional assay so the engineering and comparison studies can be scoped separately.
What should be tested after antibody engineering?
Compare the engineered material with the parent using binding and relevant functional assays. Expression, aggregation and stability may also influence whether a variant is useful. Define the comparator, acceptance criteria and requested analysis before selecting an engineering package.
What affects the cost of antibody engineering & humanisation?
Pricing depends on starting sequence or source material, number of variants, expression format and binding or functional comparisons. ABMIUM provides a project-specific quotation after reviewing the requirement. Ask for a breakdown of stages, deliverables, optional studies and further-work decision points so you can compare the total scope rather than an entry price alone.
Talk to ABMIUM
Request a technical review & quote
Share the information below to help us scope antibody engineering & humanisation.
- Existing clone, cell line or sequence and any known chain information.
- Target antigen and baseline binding or functional evidence.
- Desired format, species, isotype, tags and quantity.
- Comparison assays, acceptance criteria and sequence ownership requirements.
info@abmium.com
+44 (0)203 824 9119
UK-based ABMIUM supports research enquiries from the UK, Europe and the United States. Include your destination country so material transfer, delivery and documentation requirements can be assessed. Research Use Only; feasibility and lead time are confirmed against the final specification.