Recombinant Human Bcl-xL protein

Background

Bcl-xL is a transmembrane protein located in the mitochondrial membranes of cells that are long-lived and postmitotic, such as adult brain cells. It plays a role in the signal transduction pathway of the FAS-Ligand. Bcl-xL is an anti-apoptotic protein which is a member of the Bcl-2 family which are able to form heterodimers, and this is an significant event in the regulation of apoptosis. It is involved in the survival of cancer cells. Bcl-xL is the leading monitor of apoptosis/active cell suicide. It has cell death repressor activity and therefore acts as a survival protein. The human Bcl-xL shares 97 % a.a. sequence identity with Mouse and rat Bcl-xL.

Expression system

Escherichia coli.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 23.7 kDa, a single non-glycosylated polypeptide chain containing 211 amino acids.

Endotoxin

Less than 0.1 EU/μg of rHuBcl-xL as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 5 % Trehalose.

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