Human Protein Phosphatase, Mg2+, Mn2+ Dependent 1D, PPM1D ELISA Kit

BT LAB manufacturer product image

100%
Manufactured by:BT LAB
Save 15%RUO

Human Protein Phosphatase, Mg2+, Mn2+ Dependent 1D, PPM1D ELISA Kit

Human Protein Phosphatase, Mg2+, Mn2+ Dependent 1D, PPM1D ELISA Kit

SKU:
ABM218832
Manufacturer SKU
E7328Hu
Product type
ELISA Kits
TargetPPM1D
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Involved in the negative regulation of p53 expression (PubMed: 23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed: 15870257, PubMed: 16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed: 21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PPM1D antibody. PPM1D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PPM1D Antibody is added and binds to PPM1D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPM1D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PPM1D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
p53-induced protein phosphatase 1;PP2C-delta;PPM 1D;PPM1D;Protein phosphatase 1D;Protein phosphatase 2C isoform delta;Protein phosphatase magnesium-dependent 1 delta
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.019ng/ml
Detection range
0.05-15ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
66,675 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E7328Hu
Gene symbol
PPM1D
Gene ID
8493
UniProt accession
O15297
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00