Manufactured by:BT LAB
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Human Oxidative Stress-responsive 1 Protein, OSER1 ELISA Kit

Human Oxidative Stress-responsive 1 Protein, OSER1 ELISA Kit

SKU:
ABM218510
Manufacturer SKU
E6699Hu
Product type
ELISA Kits
TargetOSER1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Chromosome 20 open reading frame 111, or C20orf111, is the hypothetical protein that in humans is encoded by the C20orf111 gene. C20orf111 is also known as Perit1 (peroxide inducible transcript 1), HSPC207, and dJ1183I21.1. It was originally located using genomic sequencing of chromosome 20. The National Center for Biotechnology Information, or NCBI, shows that it is located at q13.11 on chromosome 20, however the genome browser at the University of California-Santa Cruz (UCSC) website[8] shows that it is at location q13.12, and within a million base pairs of the adenosine deaminase locus. It was also found to have an increase in expression in cells undergoing hydrogen peroxide(H 2O 2)-induced apoptosis.[10] After analyzing the amino acid content of C20orf111, it was found to be rich in serine residues.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human OSER1 antibody. OSER1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human OSER1 Antibody is added and binds to OSER1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OSER1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human OSER1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
C20orf111;dJ1183I21.1;HSPC207;OSER1;Osr1;Oxidative stress-responsive protein 1;Oxidative stress-responsive serine-rich protein 1;Perit1;Peroxide-inducible transcript 1 protein;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.12ng/L
Detection range
5-1000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
31,779 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6699Hu
Gene symbol
OSER1
Gene ID
51526
UniProt accession
Q9NX31
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00