Human B-cell Differentiation Antigen CD72, CD72 ELISA Kit

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Human B-cell Differentiation Antigen CD72, CD72 ELISA Kit

Human B-cell Differentiation Antigen CD72, CD72 ELISA Kit

SKU:
ABM218280
Manufacturer SKU
E6538Hu
Product type
ELISA Kits
TargetCD72
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

CD27 is a member of the tumor necrosis factor receptor superfamily. It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule, and is the target of an anti-cancer drug in clinical trials. During mouse embryonic development, specific (medium) expression levels of CD27 (in addition to high cKit, medium Gata2, and high CD31 expression levels) define the very first adult definitive hematopoietic stem cells generated in the aorta-gonad-mesonephros region. Furthermore, CD27 is expressed on both naïve and activated effector T cells as well as NK cells and activated B cells. It is a type I transmembrane protein with cysteine-rich domains, but once T cells have become activated, a soluble form of CD27 can be shed.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CD72 antibody. CD72 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD72 Antibody is added and binds to CD72 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD72 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD72. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Stem Cells
Also known as
B-cell differentiation antigen CD72;CD antigen CD72;CD72;CD72b;LYB2;Lyb-2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.25ng/ml
Detection range
0.5-100ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
40,220 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6538Hu
Gene symbol
CD72
Gene ID
971
UniProt accession
P21854
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00