Human Nucleosome Assembly Protein 1-like 4, NAP1L4 ELISA Kit

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Human Nucleosome Assembly Protein 1-like 4, NAP1L4 ELISA Kit

Human Nucleosome Assembly Protein 1-like 4, NAP1L4 ELISA Kit

SKU:
ABM217224
Manufacturer SKU
E5804Hu
Product type
ELISA Kits
TargetNAP1L4
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Nucleosome assembly protein 1-like 4 is a protein that in humans is encoded by the NAP1L4 gene. This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human NAP1L4 antibody. NAP1L4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NAP1L4 Antibody is added and binds to NAP1L4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NAP1L4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NAP1L4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
hNAP2;NAP1L4;NAP1L4b;NAP2;NAP-2;NAP2L;Nucleosome assembly protein 1-like 4;Nucleosome assembly protein 2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.09ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
42,823 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5804Hu
Gene symbol
NAP1L4
Gene ID
4676
UniProt accession
Q99733
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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