Manufactured by:BT LAB
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Human Long-chain Fatty Acid Transport Protein 1, SLC27A1 ELISA Kit

Human Long-chain Fatty Acid Transport Protein 1, SLC27A1 ELISA Kit

SKU:
ABM216914
Manufacturer SKU
E5593Hu
Product type
ELISA Kits
TargetSLC27A1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Mediates the ATP-dependent import of long-chain fatty acids (LCFA) into the cell by mediating their translocation at the plasma membrane (PubMed: 28178239). Has also an acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism (By similarity). Probably involved in fatty acid transport across the blood barrier (PubMed: 21395585).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SLC27A1 antibody. SLC27A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SLC27A1 Antibody is added and binds to SLC27A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SLC27A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SLC27A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
FATP-1;Fatty acid transport protein 1;Long-chain fatty acid transport protein 1;SLC27A1;Solute carrier family 27 member 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.24ng/L
Detection range
5-1000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
71,108 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5593Hu
Gene symbol
SLC27A1
Gene ID
376497
UniProt accession
Q6PCB7
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SLC27A1solute carrier family 27 member 1

View target guide

SLC27A1 identifies solute carrier family 27 member 1 in Homo sapiens (Human). This target record connects NCBI Gene 376497, UniProtKB Q6PCB7 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
376497
UniProtKB
Q6PCB7
BT LAB
£240.93£205.00