Manufactured by:BT LAB
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Human DNA Replication Licensing Factor Mcm6, MCM6 ELISA Kit

Human DNA Replication Licensing Factor Mcm6, MCM6 ELISA Kit

SKU:
ABM216264
Manufacturer SKU
E5192Hu
Product type
ELISA Kits
TargetMCM6
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MCM6 antibody. MCM6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MCM6 Antibody is added and binds to MCM6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MCM6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MCM6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
DNA replication licensing factor MCM6;MCM 6;MCM6;p105MCM
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.85ng/L
Detection range
20-6000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
92,889 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5192Hu
Gene symbol
MCM6
Gene ID
4175
UniProt accession
Q14566
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MCM6minichromosome maintenance complex component 6

View target guide

MCM6 identifies minichromosome maintenance complex component 6 in Homo sapiens (Human). This target record connects NCBI Gene 4175, UniProtKB Q14566 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
4175
UniProtKB
Q14566
BT LAB
£240.93£205.00