Manufactured by:BT LAB
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Human Aldehyde Dehydrogenase Family 1 Member A3, ALDH1A3 ELISA Kit

Human Aldehyde Dehydrogenase Family 1 Member A3, ALDH1A3 ELISA Kit

SKU:
ABM215904
Manufacturer SKU
E4919Hu
Product type
ELISA Kits
TargetALDH1A3
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid (PubMed:27759097). Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde (PubMed:27759097). Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ALDH1A3 antibody. ALDH1A3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ALDH1A3 Antibody is added and binds to ALDH1A3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ALDH1A3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ALDH1A3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Neuroscience
Also known as
Aldehyde dehydrogenase 6;Aldehyde dehydrogenase family 1 member A3;ALDH1A3;ALDH1A6;ALDH6;MCOP8;RALDH3;RALDH-3;Retinaldehyde dehydrogenase 3;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.31ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
56,108 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4919Hu
Gene symbol
ALDH1A3
Gene ID
220
UniProt accession
P47895
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00