Human Udp-Glucose 4-Epimerase, GALE ELISA Kit

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Human Udp-Glucose 4-Epimerase, GALE ELISA Kit

Human Udp-Glucose 4-Epimerase, GALE ELISA Kit

SKU:
ABM215640
Manufacturer SKU
E4621Hu
Product type
ELISA Kits
TargetGALE
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human GALE antibody. GALE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GALE Antibody is added and binds to GALE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GALE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GALE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Galactowaldenase;GALE;UDP-galactose 4-epimerase;UDP-GalNAc 4-epimerase;UDP-GlcNAc 4-epimerase;UDP-glucose 4-epimerase;UDP-N-acetylgalactosamine 4-epimerase;UDP-N-acetylglucosamine 4-epimerase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
8.07ng/L
Detection range
15-3000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
38,282 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4621Hu
Gene symbol
GALE
Gene ID
2582
UniProt accession
Q14376
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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