Manufactured by:BT LAB
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Human Plasminogen Activator Inhibitor 2, PAI-2 ELISA Kit

Human Plasminogen Activator Inhibitor 2, PAI-2 ELISA Kit

SKU:
ABM215262
Manufacturer SKU
E4027hu
Product type
ELISA Kits
TargetSERPINB2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SERPINB2 antibody. SERPINB2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SERPINB2 Antibody is added and binds to SERPINB2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SERPINB2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SERPINB2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
Monocyte Arg-serpin;PAI-2;Placental plasminogen activator inhibitor;Plasminogen activator inhibitor 2;Serpin B2;SERPINB 2;SERPINB2;Urokinase inhibitor
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
7.66ng/mL
Detection range
15-240ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
46,596 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4027hu
Gene symbol
SERPINB2
Gene ID
5055
UniProt accession
P05120
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SERPINB2serpin family B member 2

View target guide

SERPINB2 identifies serpin family B member 2 in Homo sapiens (Human). This target record connects NCBI Gene 5055, UniProtKB P05120 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5055
UniProtKB
P05120
BT LAB
£240.93£205.00