Manufactured by:BT LAB
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Human Interleukin 18 Receptor Accessory Protein, IL-18RAP ELISA Kit

Human Interleukin 18 Receptor Accessory Protein, IL-18RAP ELISA Kit

SKU:
ABM215238
Manufacturer SKU
E3992Hu
Product type
ELISA Kits
TargetIL18RAP
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation (PubMed: 9792649, PubMed: 14528293, PubMed: 25500532). May play a role in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (Probable).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL18RAP antibody. IL18RAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL18RAP Antibody is added and binds to IL18RAP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL18RAP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL18RAP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Accessory protein-like;AcPL;CD antigen CD218b;CD218 antigen-like family member B;CDw218b;IL 18RAP;IL-18 receptor accessory protein;IL-18RAcP;IL18RAP;IL-18Rbeta;IL-18R-beta;IL-1R accessory protein-like;IL-1R7;IL-1R-7;IL-1RAcPL;Interleukin-1 receptor 7;Interleukin-18 receptor accessory protein;Interleukin-18 receptor accessory protein-like;Interleukin-18 receptor beta
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.022ng/ml
Detection range
0.05-20ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
68,310 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3992Hu
Gene symbol
IL18RAP
Gene ID
8807
UniProt accession
O95256
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

IL18RAPinterleukin 18 receptor accessory protein

View target guide

IL18RAP identifies interleukin 18 receptor accessory protein in Homo sapiens (Human). This target record connects NCBI Gene 8807, UniProtKB O95256 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
8807
UniProtKB
O95256
BT LAB
£240.93£205.00