Manufactured by:BT LAB
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Human Cyclin-dependent Kinase 20, CCRK ELISA Kit

Human Cyclin-dependent Kinase 20, CCRK ELISA Kit

SKU:
ABM215144
Manufacturer SKU
E3894Hu
Product type
ELISA Kits
TargetCDK20
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing GLI2 to be properly activated in response to SHH signaling (By similarity). Involved in cell growth. Activates CDK2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160'.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CDK20 antibody. CDK20 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CDK20 Antibody is added and binds to CDK20 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CDK20 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CDK20. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
CAK-kinase p42;CDK 20;CDK20;CDK-activating kinase p42;Cell cycle-related kinase;Cell division protein kinase 20;Cyclin-dependent kinase 20;Cyclin-dependent protein kinase H;Cyclin-kinase-activating kinase p42;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
10.25ng/ml
Detection range
20-7000ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
38,695 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3894Hu
Gene symbol
CDK20
Gene ID
23552
UniProt accession
Q8IZL9
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00