Human Hepatoma Derived Growth Factor, HDGF ELISA Kit

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Human Hepatoma Derived Growth Factor, HDGF ELISA Kit

Human Hepatoma Derived Growth Factor, HDGF ELISA Kit

SKU:
ABM214996
Manufacturer SKU
E3664Hu
Product type
ELISA Kits
TargetHDGF
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

[Isoform 1]: Acts as a transcriptional repressor (PubMed: 17974029). Has mitogenic activity for fibroblasts (PubMed: 11751870, PubMed: 26845719). Heparin-binding protein (PubMed: 15491618). [Isoform 2]: Does not have mitogenic activity for fibroblasts (PubMed: 26845719). Does not bind heparin (PubMed: 26845719). [Isoform 3]: Has mitogenic activity for fibroblasts (PubMed: 26845719). Heparin-binding protein (PubMed: 26845719).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human HDGF antibody. HDGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HDGF Antibody is added and binds to HDGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HDGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human HDGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cancer
Also known as
HDGF;Hepatoma-derived growth factor;High mobility group protein 1-like 2;HMG-1L2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.031ng/ml
Detection range
0.05-30ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
26,788 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3664Hu
Gene symbol
HDGF
Gene ID
3068
UniProt accession
P51858
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

HDGFheparin binding growth factor

View target guide

HDGF identifies heparin binding growth factor in Homo sapiens (Human). This target record connects NCBI Gene 3068, UniProtKB P51858 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3068
UniProtKB
P51858
Browse all HDGF productsRead target context & evidence
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