Human Neurogenic Locus Notch Homolog Protein 2, NOTCH2 ELISA Kit

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Human Neurogenic Locus Notch Homolog Protein 2, NOTCH2 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 2, NOTCH2 ELISA Kit

SKU:
ABM214618
Manufacturer SKU
E3262Hu
Product type
ELISA Kits
TargetNOTCH2
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed: 21378985, PubMed: 21378989). Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed: 29149593). Positively regulates self-renewal of liver cancer cells (PubMed: 25985737).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human NOTCH2 antibody. NOTCH2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NOTCH2 Antibody is added and binds to NOTCH2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOTCH2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NOTCH2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
hN2;N2ECD;N2ICD;Neurogenic locus notch homolog protein 2;NOTCH 2;Notch 2 extracellular truncation;Notch 2 intracellular domain;NOTCH2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.26ng/ml
Detection range
0.5-100ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
265,405 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3262Hu
Gene symbol
NOTCH2
Gene ID
4853
UniProt accession
Q04721
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00