Manufactured by:BT LAB
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Human Galectin 1, GAL1 ELISA Kit

Human Galectin 1, GAL1 ELISA Kit

SKU:
ABM214328
Manufacturer SKU
E2989Hu
Product type
ELISA Kits
TargetLGALS1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human LGALS1 antibody. LGALS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LGALS1 Antibody is added and binds to LGALS1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LGALS1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LGALS1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology
Also known as
14 kDa laminin-binding protein;14 kDa lectin;Beta-galactoside-binding lectin L-14-I;Gal-1;Galaptin;Galectin-1;HBL;HLBP14;HPL;Lactose-binding lectin 1;Lectin galactoside-binding soluble 1;LGALS 1;LGALS1;Putative MAPK-activating protein PM12;S-Lac lectin 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.15ng/ml
Detection range
0.3-90ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
14,716 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2989Hu
Gene symbol
LGALS1
Gene ID
3956
UniProt accession
P09382
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

LGALS1galectin 1

View target guide

LGALS1 identifies galectin 1 in Homo sapiens (Human). This target record connects NCBI Gene 3956, UniProtKB P09382 and related ABMIUM catalogue products.

Biological function: LGALS1 (galectin-1) is a beta-galactoside-binding lectin that regulates apoptosis, cell proliferation and differentiation. It can modulate CD45 phosphatase activity and T-cell responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3956
UniProtKB
P09382
BT LAB
£240.93£205.00