Human Interferon Regulatory Factor 9, IRF9 ELISA Kit

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Human Interferon Regulatory Factor 9, IRF9 ELISA Kit

Human Interferon Regulatory Factor 9, IRF9 ELISA Kit

SKU:
ABM214000
Manufacturer SKU
E2760Hu
Product type
ELISA Kits
TargetIRF9
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IRF9 antibody. IRF9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IRF9 Antibody is added and binds to IRF9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IRF9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IRF9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
IFN-alpha-responsive transcription factor subunit;Interferon regulatory factor 9;Interferon-stimulated gene factor 3 gamma;IRF 9;IRF9;IRF-9;ISGF-3 gamma;ISGF3 p48 subunit;Transcriptional regulator ISGF3 subunit gamma;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.58ng/L
Detection range
20-3800ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
43,696 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2760Hu
Gene symbol
IRF9
Gene ID
10379
UniProt accession
Q00978
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00