Mouse Sphingosine-1-phosphate Lyase 1, SGPL1 ELISA Kit

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Mouse Sphingosine-1-phosphate Lyase 1, SGPL1 ELISA Kit

Mouse Sphingosine-1-phosphate Lyase 1, SGPL1 ELISA Kit

SKU:
ABM213326
Manufacturer SKU
E2486Mo
Product type
ELISA Kits
TargetSGPL1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine (PubMed:9464245, PubMed:20097939). Elevates stress-induced ceramide production and apoptosis (PubMed:9464245). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts (PubMed:20097939, PubMed:28262793). Involved in the regulation of pro-inflammatory response and neutrophil trafficking (PubMed:21173151). Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (PubMed:28521611). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse SGPL1 antibody. SGPL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SGPL1 Antibody is added and binds to SGPL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SGPL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse SGPL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
AI428538;D10Xrf45;D10Xrf456;mSPL;S1PL;SGPL1;Sphingosine-1-phosphate aldolase;Sphingosine-1-phosphate lyase 1;Spl;SPL 1;SP-lyase 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
3.44ng/L
Detection range
7-1500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
63,677 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2486Mo
Gene symbol
Sgpl1
Gene ID
20397
UniProt accession
Q8R0X7
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00