Mouse Neudesin, NENF ELISA Kit

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Mouse Neudesin, NENF ELISA Kit

Mouse Neudesin, NENF ELISA Kit

SKU:
ABM212640
Manufacturer SKU
E2288Mo
Product type
ELISA Kits
TargetNENF
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Acts as a neurotrophic factor in postnatal mature neurons, enhancing neuronal survival (PubMed: 15605373, PubMed: 31536960). Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocytes (PubMed: 16547973). Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways (PubMed: 15605373, PubMed: 16547973). Neurotrophic activity is enhanced by binding to heme (PubMed: 18056703). Acts also as an anorexigenic neurotrophic factor that contributes to energy balance (PubMed: 23576617).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse NENF antibody. NENF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NENF Antibody is added and binds to NENF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NENF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NENF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
NENF;Neudesin;Neuron-derived neurotrophic factor;Secreted protein of unknown function;SPUF protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.53ng/L
Detection range
1-400ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
18,904 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2288Mo
Gene symbol
NENF
Gene ID
66208
UniProt accession
Q9CQ45
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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