Manufactured by:BT LAB
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Bovine Appetite-Regulating Hormone, GHRL ELISA Kit

Bovine Appetite-Regulating Hormone, GHRL ELISA Kit

SKU:
ABM212092
Manufacturer SKU
E2187Bo
Product type
ELISA Kits
TargetGHRL
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity). Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine GHRL antibody. GHRL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine GHRL Antibody is added and binds to GHRL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GHRL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine GHRL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Appetite-regulating hormone;Ghrelin;GHRL;Growth hormone secretagogue;Growth hormone-releasing Peptide;Motilin-related Peptide;Obestatin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.16ng/L
Detection range
0.3-90ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
12,793 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2187Bo
Gene symbol
GHRL
Gene ID
281192
UniProt accession
Q9BDJ6
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

GHRLghrelin and obestatin prepropeptide

View target guide

GHRL identifies ghrelin and obestatin prepropeptide in Homo sapiens (Human). This target record connects NCBI Gene 51738, UniProtKB Q9UBU3 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
51738
UniProtKB
Q9UBU3
BT LAB
£344.83£293.00