Manufactured by:BT LAB
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Bovine Alpha-2-Hs-glycoprotein, AHSG ELISA Kit

Bovine Alpha-2-Hs-glycoprotein, AHSG ELISA Kit

SKU:
ABM212084
Manufacturer SKU
E2186Bo
Product type
ELISA Kits
TargetAHSG
ApplicationsELISA
Species / reactivityBovine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine AHSG antibody. AHSG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine AHSG Antibody is added and binds to AHSG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AHSG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine AHSG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
AHSG;Alpha-2-HS-glycoprotein;Asialofetuin;Fetuin-A
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
30.25ng/mL
Detection range
60-18000ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
38,419 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2186Bo
Gene symbol
AHSG
Gene ID
280988
UniProt accession
P12763
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AHSGalpha 2-HS glycoprotein

View target guide

AHSG encodes alpha-2-HS-glycoprotein (fetuin-A), a secreted serum glycoprotein synthesized by hepatocytes and concentrated in bone matrix. The AHSG/fetuin-A target is studied in mineral handling, calcification, bone biology and metabolic or inflammatory physiology.

Biological function: Alpha-2-HS-glycoprotein (AHSG; fetuin-A) is a secreted plasma glycoprotein with calcium-binding and opsonic properties. Reviewed UniProt annotations support roles in endocytosis, influence on the mineral phase of bone and regulation of bone mineralization.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
197
UniProtKB
P02765
BT LAB
£240.93£205.00