Rat Kallikrein 1, KLK 1 ELISA Kit

BT LAB manufacturer product image

100%
Manufactured by:BT LAB
Save 15%RUO

Rat Kallikrein 1, KLK 1 ELISA Kit

Rat Kallikrein 1, KLK 1 ELISA Kit

SKU:
ABM211860
Manufacturer SKU
E2122Ra
Product type
ELISA Kits
TargetKLK 1
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Kallikrein-1 is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. This protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat KLK 1 antibody. KLK 1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat KLK 1 Antibody is added and binds to KLK 1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KLK 1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat KLK 1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
Gamma-NGF;Kallikrein-1;KLK 1;Klk1;Klk1c1;Klna4;Nerve growth factor gamma chain;Nerve growth factor gamma chain 1;Nerve growth factor gamma chain 2];Ngfg;Pancreatic kallikrein;PS kallikrein;rGK-1;rK-1;Tissue kallikrein;True kallikrein;True tissue kallikrein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.213ng/mL
Detection range
0.5-100ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
28,852 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2122Ra
Gene symbol
Ngfg
Gene ID
24594
UniProt accession
P00758
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00