Manufactured by:BT LAB
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Mouse Cd82 Antigen, CD82 ELISA Kit

Mouse Cd82 Antigen, CD82 ELISA Kit

SKU:
ABM211252
Manufacturer SKU
E1922Mo
Product type
ELISA Kits
TargetCD82
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

CD82 (Cluster of Differentiation 82), or KAI1, is a human protein encoded by the CD82 gene. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the tetraspanin/transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CD82 antibody. CD82 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CD82 Antibody is added and binds to CD82 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD82 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CD82. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Epigenetics and Nuclear Signaling, Cancer
Also known as
AL023070;C33;C33 antigen;CD antigen CD82;CD82;CD82 antigen;IA4;Inducible membrane protein R2;Ka;Kai1;Metastasis suppressor Kangai-1 homolog;Tsp;Tspan27;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.55ng/L
Detection range
3-900ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
29,629 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1922Mo
Gene symbol
Cd82
Gene ID
12521
UniProt accession
P40237
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00