Manufactured by:BT LAB
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Rat Actin, Cytoplasmic 1, ACTB ELISA Kit

Rat Actin, Cytoplasmic 1, ACTB ELISA Kit

SKU:
ABM211022
Manufacturer SKU
E1841Ra
Product type
ELISA Kits
TargetACTB
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ACTB antibody. ACTB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ACTB Antibody is added and binds to ACTB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACTB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ACTB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
ACTB;Actin, cytoplasmic 1;Actin, cytoplasmic 1, N-terminally processed;Beta-actin
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.019ng/mL
Detection range
0.05-20ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
41,737 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1841Ra
Gene symbol
ACTB
Gene ID
81822
UniProt accession
P60711
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ACTBactin beta

View target guide

Actin beta (ACTB) encodes actin, cytoplasmic 1, commonly called beta-actin or β-actin, a widely expressed non-muscle cytoskeletal actin. Human ACTB maps to NCBI Gene 60 and reviewed UniProtKB P60709; this target record links the verified target to related ABMIUM catalogue products.

Biological function: ACTB is a highly conserved actin that polymerises from monomeric G-actin into F-actin filaments and cross-linked cytoskeletal networks. NCBI and reviewed UniProt describe roles in cell structure, motility and contraction; nuclear G- and F-actin also contribute to transcriptional regulation and repair of damaged DNA.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
60
UniProtKB
P60709
BT LAB
£240.93£205.00