Manufactured by:BT LAB
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Human Nuclear Respiratory Factor 1, NRF1 ELISA Kit

Human Nuclear Respiratory Factor 1, NRF1 ELISA Kit

SKU:
ABM210864
Manufacturer SKU
E1790Hu
Product type
ELISA Kits
TargetNRF1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human NRF1 antibody. NRF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NRF1 Antibody is added and binds to NRF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NRF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NRF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Epigenetics and Nuclear Signaling
Also known as
Alpha palindromic-binding protein;Alpha-pal;NRF 1;NRF1;NRF-1;Nuclear respiratory factor 1
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.58ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
53,541 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1790Hu
Gene symbol
NRF1
Gene ID
4899
UniProt accession
Q16656
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

NRF1nuclear respiratory factor 1

View target guide

NRF1 identifies nuclear respiratory factor 1 in Homo sapiens (Human). This target record connects NCBI Gene 4899, UniProtKB Q16656 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4899
UniProtKB
Q16656
BT LAB
£240.93£205.00