Manufactured by:BT LAB
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Rat Mucin-2, MUC2 ELISA Kit

Rat Mucin-2, MUC2 ELISA Kit

SKU:
ABM210830
Manufacturer SKU
E1770Ra
Product type
ELISA Kits
TargetMUC2
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MUC2 antibody. MUC2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MUC2 Antibody is added and binds to MUC2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MUC2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MUC2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Tags & Cell Markers
Also known as
Fragment;HH-Muc;Intestinal mucin-2;MLP;MUC2;MUC-2;Mucin-2
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.25ng/ml
Detection range
0.5-100ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
166,038 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1770Ra
Gene symbol
Muc2
Gene ID
24572
UniProt accession
Q62635
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

MUC2mucin 2, oligomeric mucus/gel-forming

View target guide

MUC2 identifies mucin 2, oligomeric mucus/gel-forming in Homo sapiens (Human). This target record connects NCBI Gene 4583, UniProtKB Q02817 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4583
UniProtKB
Q02817
BT LAB
£240.93£205.00