Human Netrin-1, NTN1 ELISA Kit

SKU:: ABM210024
Manufacturer SKU: E1277Hu
RRID
Product type: ELISA Kits

TargetNTN1

ApplicationsELISA

Species / reactivityHuman

Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:: BT LAB
Sold by:: ABMIUM
Fulfilled from:: Manufacturer facility or confirmed fulfilment location

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Product description

Background

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed: 28483977). Involved in dorsal root ganglion axon projection towards the spinal cord (PubMed: 28483977). It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis (PubMed: 15343335).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human NTN1 antibody. NTN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NTN1 Antibody is added and binds to NTN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NTN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NTN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Key facts & specifications

Research areas: Neuroscience
Also known as: Epididymis tissue protein Li 131P;Netrin-1;NTN 1;NTN1
Assay type: Sandwich
Detection method: Colorimetric (450 nm)
Sensitivity: 5.39pg/ml
Detection range: 10-3000pg/ml
Plate format: Pre-coated ELISA plate
Assay duration: 1h 30m
Molecular weight: 67,748 Da
Quantity per pack: 48T, 96T

Validation & performance evidence

ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

Evidence	Serum	Plasma	Cell culture supernatants	Ascites	Tissue homogenates
Compatibility

Identifiers & database references

Manufacturer SKU: E1277Hu
Gene symbol: NTN1
Gene ID: 9423
UniProt accession: O95631

Storage, stability & shipping

Shelf life: Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions: 2-8ºC

Compliance & documents

Datasheet: Open datasheet
Product manual: Open manual

Frequently asked questions

Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Delivery to Royaume-Uni: typically 1 to 3 business days in transit Transit estimate after dispatch. Final shipping options are confirmed at checkout.