Manufactured by:BT LAB
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Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

SKU:
ABM209806
Manufacturer SKU
E1147Mo
Product type
ELISA Kits
TargetCYP2E1
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CYP2E1 antibody. CYP2E1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CYP2E1 Antibody is added and binds to CYP2E1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CYP2E1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CYP2E1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
4-nitrophenol 2-hydroxylase;CYP 2E1;CYP2E1;CYPIIE1;Cytochrome P450 2E1;Cytochrome P450-ALC;Cytochrome P450-J
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.093ng/ml
Detection range
0.2-60ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
56,805 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1147Mo
Gene symbol
CYP2E1
Gene ID
13106
UniProt accession
Q05421
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CYP2E1cytochrome P450 family 2 subfamily E member 1

View target guide

CYP2E1 identifies cytochrome P450 family 2 subfamily E member 1 in Homo sapiens (Human). This target record connects NCBI Gene 1571, UniProtKB P05181 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1571
UniProtKB
P05181
BT LAB
£240.93£205.00