Manufactured by:BT LAB
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Porcine Granulocyte-macrophage Colony-stimulating Factor, CSF2 ELISA Kit

Porcine Granulocyte-macrophage Colony-stimulating Factor, CSF2 ELISA Kit

SKU:
ABM208876
Manufacturer SKU
E0564Po
Product type
ELISA Kits
TargetCSF2
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine CSF2 antibody. CSF2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine CSF2 Antibody is added and binds to CSF2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CSF2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine CSF2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Developmental Biology
Also known as
Colony-stimulating factor;CSF;CSF 2;CSF2;GM-CSF;Granulocyte-macrophage colony-stimulating factor
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.39ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
16,254 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0564Po
Gene symbol
CSF2
Gene ID
397208
UniProt accession
Q29118
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CSF2colony stimulating factor 2

View target guide

CSF2 identifies colony stimulating factor 2 in Homo sapiens (Human). This target record connects NCBI Gene 1437, UniProtKB P04141 and related ABMIUM catalogue products.

Biological function: CSF2 (GM-CSF) is a cytokine that stimulates growth and differentiation of haematopoietic precursor cells, including granulocyte, macrophage and eosinophil lineages.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1437
UniProtKB
P04141
BT LAB
£240.93£205.00