Manufactured by:BT LAB
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Porcine Granulocyte Colony-stimulating Factor, CSF3 ELISA Kit

Porcine Granulocyte Colony-stimulating Factor, CSF3 ELISA Kit

SKU:
ABM208874
Manufacturer SKU
E0563Po
Product type
ELISA Kits
TargetCSF3
ApplicationsELISA
Species / reactivityPorcine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine CSF3 antibody. CSF3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine CSF3 Antibody is added and binds to CSF3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CSF3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine CSF3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Cancer
Also known as
CSF 3;CSF3;G-CSF;Granulocyte colony-stimulating factor
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.54ng/L
Detection range
5-1000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
21,214 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0563Po
Gene symbol
CSF3
Gene ID
396839
UniProt accession
O02837
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CSF3colony stimulating factor 3

View target guide

Colony stimulating factor 3 (CSF3), commonly known as granulocyte colony-stimulating factor (G-CSF), is a cytokine involved in granulocyte production and function. This hub connects verified names and identifiers with ABMIUM catalogue products.

Biological function: Colony stimulating factor 3 (CSF3/G-CSF) is a secreted cytokine in the IL-6 cytokine superfamily. NCBI describes it as controlling the production, differentiation and function of granulocytes, supporting research into granulopoiesis and innate immune biology.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
1440
UniProtKB
P09919
BT LAB
£240.93£205.00