Human Lipolysaccharide Binding Protein, LBP ELISA Kit

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Human Lipolysaccharide Binding Protein, LBP ELISA Kit

Human Lipolysaccharide Binding Protein, LBP ELISA Kit

SKU:
ABM208530
Manufacturer SKU
E0360Hu
Product type
ELISA Kits
TargetLBP
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed: 7517398, PubMed: 24120359). Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed: 7517398, PubMed: 24120359).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human LBP antibody. LBP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LBP Antibody is added and binds to LBP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LBP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human LBP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
LBP;Lipopolysaccharide-binding protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.12ng/ml
Detection range
0.2-60ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
53,384 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0360Hu
Gene symbol
LBP
Gene ID
3929
UniProt accession
P18428
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

LBPlipopolysaccharide binding protein

View target guide

LBP identifies lipopolysaccharide binding protein in Homo sapiens (Human). This target record connects NCBI Gene 3929, UniProtKB P18428 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3929
UniProtKB
P18428
Browse all LBP productsRead target context & evidence
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