Manufactured by:BT LAB
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Mouse Tissue Factor Pathway Inhibitor, TFPI ELISA Kit

Mouse Tissue Factor Pathway Inhibitor, TFPI ELISA Kit

SKU:
ABM208204
Manufacturer SKU
E0268Mo
Product type
ELISA Kits
TargetTFPI
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse TFPI antibody. TFPI present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse TFPI Antibody is added and binds to TFPI in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TFPI antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse TFPI. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
EPI;Extrinsic pathway inhibitor;LACI;Lipoprotein-associated coagulation inhibitor;TFPI;Tissue factor pathway inhibitor
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
18.28ng/L
Detection range
40-640ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
34,987 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0268Mo
Gene symbol
TFPI
Gene ID
21788
UniProt accession
O54819
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

TFPItissue factor pathway inhibitor

View target guide

TFPI identifies tissue factor pathway inhibitor in Homo sapiens (Human). This target record connects NCBI Gene 7035, UniProtKB P10646 and related ABMIUM catalogue products.

Biological function: Tissue factor pathway inhibitor (TFPI; LACI/EPI) is a secreted Kunitz-type serine protease inhibitor that restrains initiation of the extrinsic coagulation pathway by inhibiting factor Xa and the tissue factor-factor VIIa complex.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7035
UniProtKB
P10646
BT LAB
£240.93£205.00