Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase, CTDP1 ELISA Kit

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Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase, CTDP1 ELISA Kit

Human Rna Polymerase Ii Subunit A C-terminal Domain Phosphatase, CTDP1 ELISA Kit

SKU:
ABM218236
Manufacturer SKU
E6507Hu
Product type
ELISA Kits
TargetCTDP1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CTDP1 antibody. CTDP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CTDP1 Antibody is added and binds to CTDP1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CTDP1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CTDP1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction, Epigenetics and Nuclear Signaling
Also known as
CCFDN;CTDP1;FCP1;RNA polymerase II subunit A C-terminal domain phosphatase;TFIIF-associating CTD phosphatase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
9.32ng/L
Detection range
20-4500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
104,399 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6507Hu
Gene symbol
CTDP1
Gene ID
9150
UniProt accession
Q9Y5B0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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