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Free ELISA Data Analyser and Standard Curve Tool

Calculate ELISA standard curves, analyse duplicate OD readings, review CV values and estimate sample concentrations from a 96-well plate, all in your browser.

  • Sandwich, competitive, direct and indirect ELISA
  • Standard-curve fitting with R² reporting
  • Plate layout, dilution and replicate analysis
  • Excel and PDF export
ABMIUM
ELISA Analyser
Sandwich
Standard Series
Enter known concentrations & duplicate OD readings
IDConcOD Rep 1OD Rep 2Avg ODCorr. OD
Standard Curve
x = Corrected OD · y = Concentration
No curve data
Enter at least 3 standards with OD values
96-Well Plate Editor
Click a well · Tab/Enter to advance · Arrow keys to navigate
Assigning sample names
Standard
Blank
Sample
Core Definitions
Corrected OD
Sandwich/Direct/Indirect: Avg OD − Blank Avg OD. Competitive: Raw Avg OD (no subtraction).
Standard Curve
2nd order polynomial: Conc = a·OD² + b·OD + c. R² must exceed 0.990 for valid results.
CV%
SD of replicate concentrations ÷ mean × 100. CV% <15% acceptable. CV% >20% indicates variability.
OOR (Out of Range)
Sample OD outside the standard curve range. Re-assay at a different dilution.
ELISA Format Comparison
FormatSignal vs ConcBlank Correction
SandwichIncreases ↑Subtract blank OD
DirectIncreases ↑Subtract blank OD
IndirectIncreases ↑Subtract blank OD
CompetitiveDecreases ↓Raw OD (no subtraction)
Settings
Assay Information
Assay / Project Name
Analyst Name
Run Date
Notes
Assay Configuration
ELISA Type
Concentration Units
Acceptable CV% Threshold
Chart Appearance
Fitted curve colour
Standard point colour
Point size - 5px
Show grid lines
Show equation on chart
Data Management