Custom research services · ABMIUM
Bispecific Antibody Development
Develop custom bispecific antibodies for research. Discuss target combinations, molecular formats, expression, purification and dual-binding assessment.
Specification reviewed before feasibility, pricing and lead time are confirmed.
Built around the experiment
Your project. A defined technical scope.
A bispecific antibody combines recognition of two targets in a single engineered molecule. ABMIUM coordinates custom bispecific development from parental antibody sequences or an agreed discovery route. The project should connect format design to expression, purification and assessment of both binding arms. For immune-cell engager research, a functional assay plan is as important as demonstrating antigen binding.
Specialist ProMab service routes can be coordinated through ABMIUM. Your quotation identifies the selected provider, agreed stages, available documentation and material or experimental deliverables.
ABMIUM reviews your specification and coordinates suitable custom-service and manufacturing routes. The proposed provider or manufacturer, available documentation and agreed scope are stated before order.
Choose the stages you need
Service modules
Start with one module or combine related stages into a clearly scoped project.
Bispecific format and sequence design
Discuss fragment-based and IgG-like formats, including BiTE, DART, CrossMAb, DVD-Ig and knobs-into-holes approaches where suitable. Supply parental sequences, antigen identities and intended...
Technical scope
Discuss fragment-based and IgG-like formats, including BiTE, DART, CrossMAb, DVD-Ig and knobs-into-holes approaches where suitable. Supply parental sequences, antigen identities and intended mechanism. Format selection should consider valency, geometry, expression and the assays available to evaluate the construct.
Pilot expression and purification
Start with an agreed expression and purification stage to assess whether the construct can be recovered in a useful form. Mammalian expression...
Technical scope
Start with an agreed expression and purification stage to assess whether the construct can be recovered in a useful form. Mammalian expression and purity assessment can be included. Define the pilot amount, buffer and material needed for the next experimental stage.
Binding assessment for both targets
Plan assessment of each binding arm using appropriate antigen-based or cell-based assays. ELISA, western blot or flow cytometry may be scoped according...
Technical scope
Plan assessment of each binding arm using appropriate antigen-based or cell-based assays. ELISA, western blot or flow cytometry may be scoped according to the antigen and sample. Binding to one target alone does not establish the performance of the complete bispecific molecule.
Research scale-up and functional testing
Progress selected constructs to a larger research expression stage and, where required, functional studies. Set the amount, acceptance criteria and analytical package...
Technical scope
Progress selected constructs to a larger research expression stage and, where required, functional studies. Set the amount, acceptance criteria and analytical package before scale-up. Cell-based assays and preclinical evaluation can be requested as linked project modules.
Connect the service to your research
Research applications
Immune-cell engager research
Explore target-cell and immune-cell binding combinations with relevant positive, negative and single-arm controls.
Dual-target biology
Investigate combined recognition or pathway questions using an agreed molecular format and assay system.
Bispecific format screening
Compare constructs against the same binding and functional criteria before choosing material for further studies.
Agree the output before work begins
Project deliverables
Material, experiments and documentation are defined in the quotation. Select the deliverables that your study needs.
- Agreed construct sequences and design information.
- Purified bispecific antibody material at the commissioned scale.
- Purity and binding data for the tests included in the project.
- A research report with experimental methods and defined next-stage requirements.
One coordinated route
How your custom project works
- 01
Share your requirement
Tell us the target, application, starting materials and output you need. Include performance and documentation requirements.
- 02
Review feasibility and scope
ABMIUM coordinates the technical review and identifies the proposed provider, methods, deliverables and project stages.
- 03
Confirm the quotation
Review the agreed specification, available QC and reports, pricing, indicative lead time and delivery terms before order.
Clear answers before you enquire
Frequently asked questions
Do I need to provide both antibody sequences?
Existing sequences can support a direct engineering route. If one or both binders need development, state that in the enquiry so discovery can be scoped separately.
Which bispecific format should I choose?
The appropriate format depends on your targets, intended mechanism, expression needs and assay system. The technical review can compare suitable options.
Is dual binding enough to establish function?
No. Binding and function answer different questions. Include the cell-based or other functional experiments needed for your research objective.
Can I commission a small pilot first?
A staged project can be discussed, with pilot expression and binding assessment preceding larger-scale material supply.
How do we choose a bispecific antibody format?
Start with the two targets, required geometry and intended mechanism. An IgG-like format and a fragment-based construct can differ in valency, size, production and behaviour. Existing binder sequences, Fc requirements and the planned assay help define which formats are worth assessing.
Does binding to two targets prove the construct works?
No. Individual binding measurements do not establish simultaneous binding or the desired biological response. Agree dual-binding experiments and mechanism-relevant functional testing, alongside identity, purity and aggregation checks.
What affects the cost of bispecific antibody development?
Pricing depends on available binder sequences, format design, number of constructs, purification and dual-binding or functional assessment. ABMIUM provides a project-specific quotation after reviewing the requirement. Ask for a breakdown of stages, deliverables, optional studies and further-work decision points so you can compare the total scope rather than an entry price alone.
Talk to ABMIUM
Request a technical review & quote
Share the information below to help us scope bispecific antibody development.
- Both targets and parental antibody sequences or discovery requirements.
- Preferred format, valency, Fc requirements and research objective.
- Available antigens, cell lines, controls and functional readouts.
- Pilot and final quantity, buffer, QC and intellectual-property requirements.
info@abmium.com
+44 (0)203 824 9119
UK-based ABMIUM supports research enquiries from the UK, Europe and the United States. Include your destination country so material transfer, delivery and documentation requirements can be assessed. Research Use Only; feasibility and lead time are confirmed against the final specification.