Human Ubiquitin-associated and Sh3 Domain-containing Protein A, UBASH3A ELISA Kit

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Human Ubiquitin-associated and Sh3 Domain-containing Protein A, UBASH3A ELISA Kit

Human Ubiquitin-associated and Sh3 Domain-containing Protein A, UBASH3A ELISA Kit

SKU:
ABM218084
Manufacturer SKU
E6375Hu
Product type
ELISA Kits
TargetUBASH3A
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human UBASH3A antibody. UBASH3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human UBASH3A Antibody is added and binds to UBASH3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated UBASH3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human UBASH3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Cbl-interacting protein 4;CLIP4;STS-2;Suppressor of T-cell receptor signaling 2;T-cell ubiquitin ligand 1;TULA;TULA-1;UBASH3A;Ubiquitin-associated and SH3 domain-containing protein A;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.14ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
74,123 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6375Hu
Gene symbol
UBASH3A
Gene ID
53347
UniProt accession
P57075
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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