Manufactured by:BT LAB
Save 15%RUO

Human Tyrosine-protein Phosphatase Non-receptor Type 6, PTPN6 ELISA Kit

Human Tyrosine-protein Phosphatase Non-receptor Type 6, PTPN6 ELISA Kit

SKU:
ABM218072
Manufacturer SKU
E6366Hu
Product type
ELISA Kits
TargetPTPN6
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PTPN6 antibody. PTPN6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTPN6 Antibody is added and binds to PTPN6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PTPN6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PTPN6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cell Biology, Signal Transduction, Cancer
Also known as
HCP;HCPH;Hematopoietic cell protein-tyrosine phosphatase;HPTP1C;Protein-tyrosine phosphatase 1C;Protein-tyrosine phosphatase SHP-1;PTP-1C;PTPN6;SHP1;SHP-1;SH-PTP1;Tyrosine-protein phosphatase non-receptor type 6
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
5.18ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
67,561 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6366Hu
Gene symbol
PTPN6
Gene ID
5777
UniProt accession
P29350
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PTPN6protein tyrosine phosphatase non-receptor type 6

View target guide

PTPN6 identifies protein tyrosine phosphatase non-receptor type 6 in Homo sapiens (Human). This target record connects NCBI Gene 5777, UniProtKB P29350 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5777
UniProtKB
P29350
BT LAB
£240.93£205.00