Manufactured by:BT LAB
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Human Lysosomal Protective Protein, CTSA ELISA Kit

Human Lysosomal Protective Protein, CTSA ELISA Kit

SKU:
ABM216956
Manufacturer SKU
E5615Hu
Product type
ELISA Kits
TargetCTSA
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CTSA antibody. CTSA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CTSA Antibody is added and binds to CTSA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CTSA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CTSA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
Carboxypeptidase C;Carboxypeptidase L;Cathepsin A;CTSA;GLB2;GSL;Lysosomal protective protein;Lysosomal protective protein 20 kDa chain];Lysosomal protective protein 32 kDa chain;NGBE;PPCA;PPGB;Protective protein cathepsin A;Protective protein for beta-galactosidase
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
4.95ng/L
Detection range
10-2000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
54,466 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E5615Hu
Gene symbol
CTSA
Gene ID
5476
UniProt accession
P10619
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CTSAcathepsin A

View target guide

CTSA identifies cathepsin A in Homo sapiens (Human). This target record connects NCBI Gene 5476, UniProtKB P10619 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5476
UniProtKB
P10619
BT LAB
£240.93£205.00