Manufactured by:BT LAB
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Human Alpha-Enolase, ENO1 ELISA Kit

Human Alpha-Enolase, ENO1 ELISA Kit

SKU:
ABM215920
Manufacturer SKU
E4933Hu
Product type
ELISA Kits
TargetENO1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PubMed: 29775581, PubMed: 1369209). In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed: 2005901, PubMed: 10802057, PubMed: 12666133, PubMed: 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed: 12666133). Stimulates immunoglobulin production (PubMed: 1369209). MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human ENO1 antibody. ENO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ENO1 Antibody is added and binds to ENO1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ENO1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ENO1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Metabolism
Also known as
2-phospho-D-glycerate hydro-lyase;Alpha-enolase;C-myc promoter-binding protein;ENO 1;ENO1;Enolase 1;MBP-1;MPB-1;NNE;Non-neural enolase;Phosphopyruvate hydratase;Plasminogen-binding protein
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.048ng/ml
Detection range
0.1-40ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
47,169 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E4933Hu
Gene symbol
ENO1
Gene ID
2023
UniProt accession
P06733
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ENO1enolase 1

View target guide

ENO1 identifies enolase 1 in Homo sapiens (Human). This target record connects NCBI Gene 2023, UniProtKB P06733 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2023
UniProtKB
P06733
BT LAB
£240.93£205.00