Manufactured by:BT LAB
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Human 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Human 14-3-3 Protein Epsilon, YWHAE ELISA Kit

SKU:
ABM215020
Manufacturer SKU
E3713Hu
Product type
ELISA Kits
TargetYWHAE
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed: 12917326).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human YWHAE antibody. YWHAE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human YWHAE Antibody is added and binds to YWHAE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated YWHAE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human YWHAE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
14-3-3 protein epsilon;14-3-3E;YWHAE
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
14.77ng/L
Detection range
30-9000ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
29,174 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E3713Hu
Gene symbol
YWHAE
Gene ID
7531
UniProt accession
P62258
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

YWHAEtyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon

View target guide

YWHAE identifies tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon in Homo sapiens (Human). This target record connects NCBI Gene 7531, UniProtKB P62258 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7531
UniProtKB
P62258
BT LAB
£240.93£205.00