Manufactured by:BT LAB
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Human V-Set Domain Containing T-cell Activation Inhibitor 1, VTCN1 ELISA Kit

Human V-Set Domain Containing T-cell Activation Inhibitor 1, VTCN1 ELISA Kit

SKU:
ABM213722
Manufacturer SKU
E2620Hu
Product type
ELISA Kits
TargetVTCN1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human VTCN1 antibody. VTCN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VTCN1 Antibody is added and binds to VTCN1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VTCN1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VTCN1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
B7 homolog 4;B7h.5;B7-H4;Immune costimulatory protein B7-H4;Protein B7S1;T-cell costimulatory molecule B7x;V-set domain-containing T-cell activation inhibitor 1;VTCN 1;VTCN1;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.021ng/ml
Detection range
0.05-20ng/ml
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
30,878 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2620Hu
Gene symbol
VTCN1
Gene ID
79679
UniProt accession
Q7Z7D3
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VTCN1V-set domain containing T cell activation inhibitor 1

View target guide

VTCN1 identifies V-set domain containing T cell activation inhibitor 1 in Homo sapiens (Human). This target record connects NCBI Gene 79679, UniProtKB Q7Z7D3 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
79679
UniProtKB
Q7Z7D3
BT LAB
£240.93£205.00