Manufactured by:BT LAB
Save 15%RUO

Mouse C-C Motif Chemokine 27, CCL27 ELISA Kit

Mouse C-C Motif Chemokine 27, CCL27 ELISA Kit

SKU:
ABM213720
Manufacturer SKU
E2619Mo
Product type
ELISA Kits
TargetCCL27
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CCL27 antibody. CCL27 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CCL27 Antibody is added and binds to CCL27 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL27 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CCL27. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology, Cancer
Also known as
ALP;CC chemokine ILC;C-C motif chemokine 27;CCL27;CTACK;CTAK;Cutaneous T-cell-attracting chemokine;ESKINE;IL-11 R-alpha-locus chemokine;ILC;mILC;PESKY;Skinkine;Small-inducible cytokine A27
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
4.03ng/L
Detection range
7-1500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
13,464 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2619Mo
Gene symbol
Ccl27
Gene ID
10850
UniProt accession
Q9Z1X0
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CCL27C-C motif chemokine ligand 27

View target guide

CCL27 identifies C-C motif chemokine ligand 27 in Homo sapiens (Human). This target record connects NCBI Gene 10850, UniProtKB Q9Y4X3 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
10850
UniProtKB
Q9Y4X3
BT LAB
£240.93£205.00