Manufactured by:BT LAB
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Mouse Heat Shock 70 kDa Protein 1B, HSPA1B ELISA Kit

Mouse Heat Shock 70 kDa Protein 1B, HSPA1B ELISA Kit

SKU:
ABM211848
Manufacturer SKU
E2120Mo
Product type
ELISA Kits
TargetHSPA1B
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Heat shock 70kDa protein 1B, also known as HSPA1B, is a human gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse HSPA1B antibody. HSPA1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HSPA1B Antibody is added and binds to HSPA1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse HSPA1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Signal Transduction
Also known as
2700081N06Rik;Heat shock 70 kDa protein 12B;HSPA1B
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.214ng/mL
Detection range
0.5-100ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
76,119 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E2120Mo
Gene symbol
Hspa12b
Gene ID
72630
UniProt accession
Q9CZJ2
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00