Manufactured by:BT LAB
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Mouse Fibronectin Type 3 Domain-containing Protein 5, FNDC5 ELISA Kit

Mouse Fibronectin Type 3 Domain-containing Protein 5, FNDC5 ELISA Kit

SKU:
ABM210028
Manufacturer SKU
E1278Mo
Product type
ELISA Kits
TargetFNDC5
ApplicationsELISA
Species / reactivityMouse
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a type I transmembrane glycoprotein that is encoded by the FNDC5 gene. Irisin is a cleaved version of FNDC5, named after the Greek messenger goddess Iris. Fibronectin domain-containing protein 5 is a membrane protein comprising a short cytoplasmic domain, a transmembrane segment, and an ectodomain consisting of a ~100 kDa fibronectin type III (FNIII) domain.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse FNDC5 antibody. FNDC5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FNDC5 Antibody is added and binds to FNDC5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FNDC5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FNDC5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
1500001L03Rik;AI836596;C87088;Fibronectin type III domain-containing protein 5;Fibronectin type III repeat-containing protein 2;FNDC5;Irisin];PeP;Peroxisomal protein;Pxp
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
1.04ng/L
Detection range
2-700ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
23,321 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1278Mo
Gene symbol
Fndc5
Gene ID
384061
UniProt accession
Q8K4Z2
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FNDC5fibronectin type III domain containing 5

View target guide

FNDC5 identifies fibronectin type III domain containing 5 in Homo sapiens (Human). This target record connects NCBI Gene 252995, UniProtKB Q8NAU1 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
252995
UniProtKB
Q8NAU1
BT LAB
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