Manufactured by:BT LAB
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Rat Angiotensin 3, ANG-3 ELISA Kit

Rat Angiotensin 3, ANG-3 ELISA Kit

SKU:
ABM209836
Manufacturer SKU
E1169Ra
Product type
ELISA Kits
TargetAGT
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. [Angiotensin-2]: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. [Angiotensin-3]: stimulates aldosterone release. [Angiotensin 1-7]: is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat AGT antibody. AGT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat AGT Antibody is added and binds to AGT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AGT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat AGT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Cardiovascular
Also known as
AGT;Ang I;Ang II;Ang III;Ang IV;Angiotensin 1-10;Angiotensin 1-4;Angiotensin 1-5;Angiotensin 1-7;Angiotensin 1-8;Angiotensin 1-9;Angiotensin 2-8;Angiotensin 3-8;Angiotensin I;Angiotensin II;Angiotensin III;Angiotensin IV;Angiotensin-1;Angiotensin-2;Angiotensin-3;Angiotensin-4;Angiotensinogen;Des-Asp[1]-angiotensin II;Serpin A8
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
2.41ng/L
Detection range
5-1500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
51,982 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E1169Ra
Gene symbol
AGT
Gene ID
24179
UniProt accession
P01015
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AGTangiotensinogen

View target guide

Angiotensinogen (AGT), also known as serpin A8/SERPINA8, is the precursor protein of angiotensin peptides in the renin-angiotensin system. This ABMIUM record connects NCBI Gene 183 and UniProtKB P01019 to catalogue-backed research products.

Biological function: Angiotensinogen (AGT) is an essential precursor in the renin-angiotensin system. Renin cleavage generates angiotensin I, which is further processed to bioactive angiotensin peptides including angiotensin II, supporting regulation of blood pressure, vascular tone, body-fluid balance and electrolyte homeostasis.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
183
UniProtKB
P01019
BT LAB
£240.93£205.00