Human Mitogen-activated Protein Kinase 6, MAPK6 ELISA Kit

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Human Mitogen-activated Protein Kinase 6, MAPK6 ELISA Kit

Human Mitogen-activated Protein Kinase 6, MAPK6 ELISA Kit

SKU:
ABM209186
Manufacturer SKU
E0743Hu
Product type
ELISA Kits
TargetMAPK6
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MAPK6 antibody. MAPK6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MAPK6 Antibody is added and binds to MAPK6 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MAPK6 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MAPK6. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Others
Also known as
ERK-3;Extracellular signal-regulated kinase 3;MAP kinase 6;MAP kinase isoform p97;MAPK 6;MAPK6;Mitogen-activated protein kinase 6;p97-MAPK
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.092ng/mL
Detection range
0.2-60ng/mL
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
82,681 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0743Hu
Gene symbol
MAPK6
Gene ID
5597
UniProt accession
Q16659
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

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