Manufactured by:BT LAB
Save 15%RUO

Canine Orexin, HCRT ELISA Kit

Canine Orexin, HCRT ELISA Kit

SKU:
ABM208544
Manufacturer SKU
E0365Ca
Product type
ELISA Kits
TargetHCRT
ApplicationsELISA
Species / reactivityCanine
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Background

Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine HCRT antibody. HCRT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine HCRT Antibody is added and binds to HCRT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HCRT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine HCRT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Metabolism
Also known as
HCRT;Hcrt1;Hcrt2;Hypocretin;Hypocretin-1;Hypocretin-2;Orexin;Orexin-A;Orexin-B;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
0.27ng/L
Detection range
0.5-200ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
13,328 Da
Quantity per pack
96T, 48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E0365Ca
Gene symbol
HCRT
Gene ID
607641
UniProt accession
Q9GLF6
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

HCRThypocretin neuropeptide precursor

View target guide

HCRT identifies hypocretin neuropeptide precursor in Homo sapiens (Human). This target record connects NCBI Gene 3060, UniProtKB O43612 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3060
UniProtKB
O43612
BT LAB
£357.63£304.00